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WORD: This avoids the coupling of amino acids within the additional cycles with the membrane as an alternative of the growing peptide chain. OBSERVE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and also some of the amino teams throughout the spot areas don't form bonds with the amino acids. Be sure all of the purification buffers are at 4 °C and carry out the protein purification within the cold room to keep away from protein denaturation.
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